|
KMID : 0368419890320010023
|
|
Journal of Plant Biology
1989 Volume.32 No. 1 p.23 ~ p.32
|
|
|
Development of a Plant Transformation Vector, pKCHI
|
|
鄭相浩/Chung, Sang Ho
高炅媛/洪周奉/崔仁成/鄭兌和/Ko, Kyung Won/Hong, Choo Bong/Choi, In Sung/Chung, Tae Wah
|
|
|
Abstract
|
|
|
|
We have developed a plasmid vector, pKCHl, for the purpose of higher plant transformation. It contains the promoter region of cauliflower mosaic virus 35S transcript (P_35s) and the terminator region of nopaline synthase gene (Tnos) with unique cloning site, Bam HI and Xba Ⅰ, between them. After inserting a foreign gene at the cloning site, P_35s-foreign gene-Tnos cassette can be recovered by using a restriction enzyme Hind Ⅲ.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|